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Clinical Chemistry, Vol 30, 399-403, Copyright © 1984 by American Association for Clinical Chemistry
MJ Jongen, FC Van Ginkel, WJ van der Vijgh, S Kuiper, JC Netelenbos and P Lips
An international 19-laboratory survey was organized to compare assays for 25-hydroxyvitamin D, 24,25-dihydroxyvitamin D, and 1,25- dihydroxyvitamin D in plasma. Each participant received two ethanolic standard solutions of each metabolite and eight plasma samples. Each laboratory used its usual procedures. Mean interlaboratory coefficients of variation (CVs) for the eight plasma samples were 35%, 43%, and 52% for 25-hydroxyvitamin D, 24,25-dihydroxyvitamin D, and 1,25- dihydroxyvitamin D, respectively. Average CVs for the standard solutions were 27%, 23%, and 25%, respectively. Of the eight plasma samples, five had the same concentration for one of the metabolites. One sample was diluted to 0.6 times its original concentration and three samples were fortified with one or more of the metabolites under investigation. Fourteen of 18 laboratories (78%) could distinguish between the five unchanged samples and the modified ones with their 25- hydroxyvitamin D assay. Nine of 12 (75%) could distinguish the modified samples from the other samples with the 24,25-dihydroxyvitamin D assay. Only eight of 15 (53%) could do this their 1,25-dihydroxyvitamin D assay. Values from different laboratories evidently cannot be intercompared without making an actual comparison of the assay procedures. Furthermore, in case of clinical applications of these assays, each laboratory should establish its own reference values and should continually use an internal reference sample to assess the precision of the procedures.
The following articles in journals at HighWire Press have cited this article:
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  I. Seiden-Long and R. Vieth Evaluation of a 1,25-Dihydroxyvitamin D Enzyme Immunoassay Clin. Chem., June 1, 2007; 53(6): 1104 - 1108. [Abstract] [Full Text] [PDF]
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 P. de Fornel-Thibaud, G. Blanchard, L. Escoffier-Chateau, S. Segond, F. Guetta, D. Begon, F. Delisle, and D. Rosenberg Unusual Case of Osteopenia Associated With Nutritional Calcium and Vitamin D Deficiency in an Adult Dog J. Am. Anim. Hosp. Assoc., January 1, 2007; 43(1): 52 - 60. [Abstract] [Full Text] [PDF]
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 N. Binkley, D. Krueger, C. S. Cowgill, L. Plum, E. Lake, K. E. Hansen, H. F. DeLuca, and M. K. Drezner Assay Variation Confounds the Diagnosis of Hypovitaminosis D: A Call for Standardization J. Clin. Endocrinol. Metab., July 1, 2004; 89(7): 3152 - 3157. [Abstract] [Full Text] [PDF]
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 C. Valmadrid, C. Voorhees, B. Litt, and C. R. Schneyer Practice Patterns of Neurologists Regarding Bone and Mineral Effects of Antiepileptic Drug Therapy Arch Neurol, September 1, 2001; 58(9): 1369 - 1374. [Abstract] [Full Text] [PDF]
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 P. Lips Vitamin D Deficiency and Secondary Hyperparathyroidism in the Elderly: Consequences for Bone Loss and Fractures and Therapeutic Implications Endocr. Rev., August 1, 2001; 22(4): 477 - 501. [Abstract] [Full Text] [PDF]
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 R. Vieth Vitamin D supplementation, 25-hydroxyvitamin D concentrations, and safety Am. J. Clinical Nutrition, May 1, 1999; 69(5): 842 - 856. [Abstract] [Full Text] [PDF]
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 M. S. LeBoff, L. Kohlmeier, S. Hurwitz, J. Franklin, J. Wright, and J. Glowacki Occult Vitamin D Deficiency in Postmenopausal US Women With Acute Hip Fracture JAMA, April 28, 1999; 281(16): 1505 - 1511. [Abstract] [Full Text] [PDF]
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C. Lind, J. Chen, and I. Byrjalsen Enzyme immunoassay for measuring 25-hydroxyvitamin D3 in serum Clin. Chem., June 1, 1997; 43(6): 943 - 949. [Abstract] [Full Text] [PDF]
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