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Clinical Chemistry 30: 443-446, 1984;
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Clinical Chemistry, Vol 30, 443-446, Copyright © 1984 by American Association for Clinical Chemistry

Direct analysis for urinary protein with biuret reagent, with use of urine ultrafiltrate blanking: comparison with a manual biuret method involving trichloroacetic acid precipitation

JH Eckfeldt, MJ Kershaw and II Dahl

We describe a method for measuring urinary protein with a centrifugal analyzer. Biuret reagent is used, and blanking with an ultrafiltrate of urine eliminates interferences from the nonprotein, biuret-positive chromogens in urine. We compare results by this new method with those by a manual method in which trichloroacetic acid precipitation and biuret reagent are used. The new method shows good precision and excellent correlation (r = 0.997) with the manual method. The ease and convenience of this assay should make this a useful method for the routine clinical laboratory.


The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
G. L. Hortin and B. Meilinger
Cross-Reactivity of Amino Acids and Other Compounds in the Biuret Reaction: Interference with Urinary Peptide Measurements
Clin. Chem., August 1, 2005; 51(8): 1411 - 1419.
[Abstract] [Full Text] [PDF]


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Copyright © 1984 by the American Association for Clinical Chemistry.