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Clinical Chemistry 30: 556-559, 1984;
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Clinical Chemistry, Vol 30, 556-559, Copyright © 1984 by American Association for Clinical Chemistry

Urea, creatinine, and glucose determined in plasma and whole blood by a differential pH technique

M Ripamonti, A Mosca, E Rovida, M Luzzana, L Luzi, F Ceriotti, F Cottini and L Rossi-Bernardi

We report the conditions (buffer composition and enzyme activity) required for estimating three frequently determined analytes--urea, glucose, and creatinine--by use of an improved version of the differential pH apparatus previously described (Clin Chem 29: 80-85, 1983). For each analyte, we used only one specific enzyme, thus avoiding a chain of auxiliary and indicator reactions. The method requires about a minute for each determination in undiluted plasma or whole blood.




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Copyright © 1984 by the American Association for Clinical Chemistry.