Clinical Chemistry
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Clinical Chemistry 30: 653-657, 1984;
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Clinical Chemistry, Vol 30, 653-657, Copyright © 1984 by American Association for Clinical Chemistry

Homogeneous luminescence immunoassay for total estrogens in urine

G Messeri, AL Caldini, GF Bolelli, M Pazzagli, A Tommasi, PL Vannucchi and M Serio

We describe an homogeneous luminescence immunoassay for "total" estrogens in enzymically hydrolyzed urine from nonpregnant women. The antiserum, raised against estriol-16,17- dihemisuccinate conjugated to bovine serum albumin, specifically bound the C-19 steroids carrying the estrogen-characteristic phenolic group. 17 beta-Estradiol conjugated with aminobutylethyl isoluminol was used to monitor the immunological reaction; this conjugate was stable for at least two years. Because binding to the antiserum markedly enhances the light-producing efficiency of the tracer, no separation of bound and free antigen is necessary. Results (microgram/24 h) by this method (y) correlated well (r = 0.958) with those by a conventional fluorometric (x) method (y = 2. 51x - 2.83). The sensitivity (detection limit) is 4 micrograms/L and the precision compares well with that of commonly used RIA methods. The method appears suited to large numbers of samples, as in menstrual cycle monitoring.




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Copyright © 1984 by the American Association for Clinical Chemistry.