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Clinical Chemistry 30: 672-676, 1984;
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Clinical Chemistry, Vol 30, 672-676, Copyright © 1984 by American Association for Clinical Chemistry

Particle-counting immunoassay of human somatotropin

CE Castracane, CL Cambiaso, LA Retegui, I Gilbert, JM Ketelslegers and PL Masson

Human somatotropin was assayed by a novel automated nonradioisotopic technique, "particle-counting immunoassay," that requires a 45-min incubation and only 60 microL of 10-fold diluted sample. The principle of the assay is agglutination of antibody-coated latex particle by somatotropin, the reaction being measured by the (instrumented) counting of residual non-agglutinated particles. The dynamic range in serum extends from 0.2 to 40 micrograms/L. The between-assay CV was 12% for a concentration of 3.3 micrograms/L and 8.5% for 31.9 micrograms/L. The coefficient of correlation (r) with radioimmunoassay was 0.97. Curves for various dilutions of the macromolecular and monomeric forms of the hormone were not parallel.




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Copyright © 1984 by the American Association for Clinical Chemistry.