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Clinical Chemistry 30: 704-706, 1984;
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Clinical Chemistry, Vol 30, 704-706, Copyright © 1984 by American Association for Clinical Chemistry

Transferability studies for the AACC reference method and the IFCC method for measurement of alkaline phosphatase activity

NW Tietz, AD Rinker, C Burtis, P Duncan, K Ervin, L Ewen, M Horder, M Mathieu, C Petitclerc and D Grisley

We present the results of measurements of alkaline phosphatase activity from interlaboratory transferability studies conducted by 12 laboratories in five countries. The variability, as demonstrated by within-day precision (CV less than or equal to 2.6%), between-day precision (CV less than or equal to 3.6%), and between-lab precision (CV less than or equal to 6.3%) establishes the transferability of this method for alkaline phosphatase. Some common errors encountered in enzyme measurements that affect the accuracy and precision of the measurements are listed.




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