Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 30: 712-716, 1984;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fesmire, J. D.
Right arrow Articles by Alaupovic, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fesmire, J. D.
Right arrow Articles by Alaupovic, P.

Clinical Chemistry, Vol 30, 712-716, Copyright © 1984 by American Association for Clinical Chemistry

Use and significance of reference serum as a secondary standard for electroimmunoassay of apolipoprotein A-I

JD Fesmire, WJ McConathy and P Alaupovic

This study was designed to show the effect of different primary standards and antisera on the variability of concentrations of apolipoprotein A-I (ApoA-I) in plasma and to document the usefulness of reference sera as a secondary standard in the electroimmunoassay of this apolipoprotein. We compared the reactivities of two separate ApoA- I samples and a preparation of high-density lipoprotein (HDL3) with seven different antisera to ApoA-I. The antisera reacted differently with each of the three standards, as shown by the different slopes of their standard curves. The resulting inconsistencies in the ApoA-I values for unknown plasma samples were shown to be corrected by the use of a reference serum. When ApoA-I analyses were carried out with the reference serum as a standard, all seven antisera gave statistically indistinguishable results for concentrations of plasma ApoA-I. The use of reference sera thus represents the simplest and most convenient means of standardizing the electroimmunoassay for ApoA-I.


The following articles in journals at HighWire Press have cited this article:


Home page
 J. Clin. Endocrinol. Metab.Home page
A. B. Singh, S. Hsia, P. Alaupovic, I. Sinha-Hikim, L. Woodhouse, T. A. Buchanan, R. Shen, R. Bross, N. Berman, and S. Bhasin
The Effects of Varying Doses of T on Insulin Sensitivity, Plasma Lipids, Apolipoproteins, and C-Reactive Protein in Healthy Young Men
J. Clin. Endocrinol. Metab., January 1, 2002; 87(1): 136 - 143.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1984 by the American Association for Clinical Chemistry.