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Clinical Chemistry 30: 712-716, 1984;
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Clinical Chemistry, Vol 30, 712-716, Copyright © 1984 by American Association for Clinical Chemistry

Use and significance of reference serum as a secondary standard for electroimmunoassay of apolipoprotein A-I

JD Fesmire, WJ McConathy and P Alaupovic

This study was designed to show the effect of different primary standards and antisera on the variability of concentrations of apolipoprotein A-I (ApoA-I) in plasma and to document the usefulness of reference sera as a secondary standard in the electroimmunoassay of this apolipoprotein. We compared the reactivities of two separate ApoA- I samples and a preparation of high-density lipoprotein (HDL3) with seven different antisera to ApoA-I. The antisera reacted differently with each of the three standards, as shown by the different slopes of their standard curves. The resulting inconsistencies in the ApoA-I values for unknown plasma samples were shown to be corrected by the use of a reference serum. When ApoA-I analyses were carried out with the reference serum as a standard, all seven antisera gave statistically indistinguishable results for concentrations of plasma ApoA-I. The use of reference sera thus represents the simplest and most convenient means of standardizing the electroimmunoassay for ApoA-I.


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Copyright © 1984 by the American Association for Clinical Chemistry.