Clinical Chemistry
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Clinical Chemistry 30: 717-723, 1984;
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Clinical Chemistry, Vol 30, 717-723, Copyright © 1984 by American Association for Clinical Chemistry

Liquid-chromatographic determination of penbutolol and its principal metabolites in plasma and urine

DJ Miner, DA Binkley and LD Bechtol

We describe a sensitive, specific liquid-chromatographic determination of penbutolol and its 4-hydroxy metabolite in plasma and urine. The method involves a simple organic extraction, evaporation of the solvent, reconstitution in methanol/water, and injection into the chromatograph. Penbutolol, its metabolites, and the internal standard, propranolol, are resolved on a CN reversed-phase column and detected fluorometrically. Conjugates of penbutolol and its 4-hydroxy metabolite may be determined after a 2-h enzymic hydrolysis. Detection limits are in the range of 3 to 12 micrograms/L of plasma. The assay is reproducible and nearly free of interferences. Representative concentrations in blood and urine of normal volunteers are reported.




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Copyright © 1984 by the American Association for Clinical Chemistry.