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Clinical Chemistry 30: 735-736, 1984;
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Clinical Chemistry, Vol 30, 735-736, Copyright © 1984 by American Association for Clinical Chemistry

Silver staining of unconcentrated cerebrospinal fluid in agarose gel (Panagel) electrophoresis

PD Mehta, SP Mehta and BA Patrick

We subjected cerebrospinal fluid (CSF) from 20 patients with multiple sclerosis and 20 patients with other neurological diseases to agarose gel ( Panagel ) electrophoresis followed by staining with silver. Ten microliters of unconcentrated CSF from multiple sclerosis patients containing 0.4 to 0.8 microgram of immunoglobulin G was found to be optimum for detection of oligoclonal IgG bands, so identified by immunofixation. The band patterns for unconcentrated CSF stained with silver were almost identical to those for the same CSF concentrated 40- fold and stained with Coomassie Brilliant Blue. Silver staining thus enables the clinical laboratory to electrophorese unconcentrated CSF on commercially prepared ( Panagel ) plates.




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Copyright © 1984 by the American Association for Clinical Chemistry.