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Clinical Chemistry 30: 760-762, 1984;
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Clinical Chemistry, Vol 30, 760-762, Copyright © 1984 by American Association for Clinical Chemistry

Discrepancies between serum free triiodothyronine and free thyroxin as measured by equilibrium dialysis and analog radioimmunoassay in nonthyroidal illnesses

K Liewendahl, S Tikanoja, T Helenius and M Valimaki

We determined free triiodothyronine (FT3) and free thyroxin (FT4) in serum of patients with various nonthyroidal illnesses (NTI), by both commercial analog-type radioimmunoassays (A) and new equilibrium dialysis procedures (D). The mean FT3 was significantly lower in NTI by both techniques, but the decreases were of different magnitudes. The FT3(D):FT3(A) ratio was 1.96, vs 1.11 for healthy controls. In NTI the mean FT4(D) was somewhat increased, whereas the mean FT4(A) was much lower than normal, the above ratio being 1.70, vs 0.92 for controls. We ascribe these discrepancies, at least in part, to binding of radiolabeled T3- and T4-analog tracers to serum albumin.


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Copyright © 1984 by the American Association for Clinical Chemistry.