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Clinical Chemistry 30: 847-850, 1984;
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Clinical Chemistry, Vol 30, 847-850, Copyright © 1984 by American Association for Clinical Chemistry

High-resolution densitometry: analysis of stained albumin bands as a model for electrophoresis of serum proteins

DJ Epstein and WE Neeley

In discussing the principles of quantitative analysis in thin-layer media, we show that requirements for quantitative analysis are not satisfied when stained protein electrophoretic bands are scanned with a conventional rectangular-slit densitometer. We investigated a high- resolution densitometer based on a linear photodiode array as an alternative analytical tool, using stained electrophoretic bands of radio-labeled human serum albumin as a simplified model for results of serum protein electrophoresis. Identical protein bands scanned with both the high-resolution densitometer and a conventional densitometer were quantified with improved accuracy and precision by the new instrument. We also used the high-resolution densitometer to develop a computer model for performance characteristics of a rectangular-slit densitometer.




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Copyright © 1984 by the American Association for Clinical Chemistry.