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Clinical Chemistry 30: 851-855, 1984;
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Clinical Chemistry, Vol 30, 851-855, Copyright © 1984 by American Association for Clinical Chemistry

Liquid-chromatographic measurement of amino acids in biological samples after formation of phenylthiohydantoin derivatives

HG Biggs and LJ Gentilcore

In this method for measuring amino acids in urine, serum, and tissue, the amino acids to be assayed and the internal standard, L-norleucine, are converted to phenylthiohydantoins , isolated by organic solvent extraction, separated by reversed-phase "high-pressure" liquid chromatography, and detected by ultraviolet absorbance. The analytes are identified by retention times and quantified by comparing peak heights with that of the internal standard. The peak-height ratios vary linearly with concentrations of 50 to 500 mg/L, corresponding to the concentration range usually found in biological samples. Detection limits are 5 to 20 mg/L. Inter- and intra-assay precision (CV) varies between 1 and 26%. Average analytical recoveries range between 67 and 100%.


The following articles in journals at HighWire Press have cited this article:


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 Clin. Chem.Home page
R. Venta
Year-Long Validation Study and Reference Values for Urinary Amino Acids Using a Reversed-Phase HPLC Method
Clin. Chem., March 1, 2001; 47(3): 575 - 583.
[Abstract] [Full Text] [PDF]

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 Clin. Chem.Home page
J. Le Boucher, C. Charret, C. Coudray-Lucas, J. Giboudeau, and L. Cynober
Amino acid determination in biological fluids by automated ion-exchange chromatography: performance of Hitachi L-8500A
Clin. Chem., August 1, 1997; 43(8): 1421 - 1428.
[Abstract] [Full Text] [PDF]


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Copyright © 1984 by the American Association for Clinical Chemistry.