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Clinical Chemistry 30: 871-874, 1984;
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Clinical Chemistry, Vol 30, 871-874, Copyright © 1984 by American Association for Clinical Chemistry

Simplified determination of carboxyhemoglobin

E Beutler and C West

This spectrophotometric method for estimating carboxyhemoglobin is based on the production of a two-pigment mixture by reducing oxyhemoglobin and methemoglobin with sodium hydrosulfite . Absorbances of the pigment are measured at 420 and 432 nm. Protecting the solutions against exposure to air is unnecessary. Values are stable and reproducible when the solutions are buffered at pH 6.85.


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Copyright © 1984 by the American Association for Clinical Chemistry.