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Clinical Chemistry, Vol 30, 889-893, Copyright © 1984 by American Association for Clinical Chemistry
R Shapiro, J Chan, A Pierson, K Vaccaro and J Quick
The chemiluminescence of fluorescein derivatives is greatly enhanced when they are conjugated to proteins. We exploited this property in devising a chemiluminescence immunoassay for rubella antibodies. In this sequential "sandwich"-type assay, tubes coated with rubella antigen are used for antibody separation and goat antibody to human IgG conjugated with fluorescein isothiocyanate is used for detection of bound rubella antibodies.
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