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Clinical Chemistry 30: 896-898, 1984;
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Clinical Chemistry, Vol 30, 896-898, Copyright © 1984 by American Association for Clinical Chemistry

Effects of azotemia on results of the boronate-agarose affinity and ion- exchange methods for glycated hemoglobin

MG Scott, JW Hoffmann, VN Meltzer, BA Siegfried and KM Chan

We evaluated the effect of azotemia on results for glycated hemoglobin as measured by a boronate-agarose affinity method and an ion-exchange chromatographic procedure with saline preincubation and found a good correlation. However, values for glycated hemoglobin in samples from nondiabetic patients with various degrees of azotemia were consistently higher with the ion-exchange column procedure (mean, 8.5%) than with the boronate affinity method (mean, 6.2%). The latter method may thus be preferred for monitoring glycated hemoglobin in diabetic patients with impaired renal function.


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D. Chen, D. L. Crimmins, F. F. Hsu, F. P. Lindberg, and M. G. Scott
Hemoglobin Raleigh as the cause of a falsely increased hemoglobin A1C in an automated ion-exchange HPLC method
Clin. Chem., June 1, 1998; 44(6): 1296 - 1301.
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Copyright © 1984 by the American Association for Clinical Chemistry.