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Clinical Chemistry 30: 901-902, 1984;
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Clinical Chemistry, Vol 30, 901-902, Copyright © 1984 by American Association for Clinical Chemistry

Automated kinetic determination of angiotensin-converting enzyme in serum

A Harjanne

In this automated kinetic modification of a previous method (Anal Biochem 95: 540-548, 1979) for determining angiotensin-converting enzyme (EC 3.4.15.1), 3-(2- furylacryloyl )-L- phenylalanylglycylglycine is used as the substrate. The change in absorbance at 340 nm is used to monitor hydrolysis of the substrate. The rate of hydrolysis is roughly threefold greater than with previously reported substrates, so assay time and sensitivity are improved.


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Copyright © 1984 by the American Association for Clinical Chemistry.