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Clinical Chemistry 30: 908-910, 1984;
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Clinical Chemistry, Vol 30, 908-910, Copyright © 1984 by American Association for Clinical Chemistry

Simultaneous determination of penicillin and cephalosporin antibiotics in serum by gradient liquid chromatography

T Annesley, K Wilkerson, K Matz and D Giacherio

We describe a "high-performance" liquid-chromatographic method for simultaneously measuring various penicillin and cephalosporin antibiotics. After extraction from serum, which in general is quantitative, the drugs are separated by use of a "Bondapak phenyl" column and a gradient mobile phase. For these drugs retention times depend on the pH of the mobile phase; we present retention times under selected pH conditions.




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Copyright © 1984 by the American Association for Clinical Chemistry.