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Clinical Chemistry 30: 1163-1167, 1984;
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Clinical Chemistry, Vol 30, 1163-1167, Copyright © 1984 by American Association for Clinical Chemistry

Theoretical aspects of one-point calibration: causes and effects of some potential errors, and their dependence on concentration

GJ Kemp

The potential errors of the one-point calibration technique are described mathematically. I examine the theoretical causes and effects of analytical errors in absorbance and of errors in determining calibration concentration, and discuss techniques for minimizing their impact. The dependence of these errors on the calibration concentration and on the size of the result is considered, and some conclusions are drawn about the choice of calibration concentration.




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Copyright © 1984 by the American Association for Clinical Chemistry.