Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 30: 1213-1215, 1984;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pekary, A. E.
Right arrow Articles by Hershman, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pekary, A. E.
Right arrow Articles by Hershman, J. M.

Clinical Chemistry, Vol 30, 1213-1215, Copyright © 1984 by American Association for Clinical Chemistry

A new monoclonal-antibody two-site solid-phase immunoradiometric assay for human thyrotropin evaluated

AE Pekary and JM Hershman

We compared results with a commercial solid-phase two-site immunoradiometric assay kit for human thyrotropin in which monoclonal antibodies are used (TANDEM-R TSH; Hybritech Inc.) with those by our radioimmunoassay (J Clin Endocrinol Metab 41:676, 1975), which is optimized for measurement of low concentrations of thyrotropin. In the immunoradiometric assay a specific antibody to the beta subunit of human thyrotropin is immobilized on a polystyrene bead, and a radiolabeled monoclonal antibody directed against the alpha subunit provides a measure of bead-immobilized hormone. The mean thyrotropin concentrations in 70 euthyroid serum samples were similar in the two assays. Values for hypothyroid patients were clearly higher in both assays than values for euthyroid individuals. In commercial assays the major source of error in measurement of thyrotropin response to thyroliberin in terms of the increment over the basal concentration of thyrotropin has been systematic errors in the measurement of those basal concentrations. With the present assay, however, basal values are obtained with good precision and accuracy: CV = 2.9% at 6 milli-int. units/L (n = 48).


The following articles in journals at HighWire Press have cited this article:


Home page
 Arch Intern MedHome page
C. T. Sawin, A. Geller, M. M. Kaplan, P. Bacharach, P. W. F. Wilson, and J. M. Hershman
Low Serum Thyrotropin (Thyroid-Stimulating Hormone) in Older Persons Without Hyperthyroidism
Arch Intern Med, January 1, 1991; 151(1): 165 - 168.
[Abstract] [PDF]

Home page
 Asia Pac J Public HealthHome page
A. Ouyang, T. Su, X. Pang, and J. M. Hershman
Serum TSH, FT3, and FT4 Levels in Inhabitants of an Endemic Goiter Area in China Supplied Iodized Salt for Twenty-five Years
Asia Pac J Public Health, October 1, 1989; 3(4): 301 - 305.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1984 by the American Association for Clinical Chemistry.