Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 30: 1219-1222, 1984;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Okabe, H.
Right arrow Articles by Noma, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Okabe, H.
Right arrow Articles by Noma, A.

Clinical Chemistry, Vol 30, 1219-1222, Copyright © 1984 by American Association for Clinical Chemistry

Automated measurement of amylase isoenzymes with 4-nitrophenyl- maltoheptaoside as substrate and use of a selective amylase inhibitor

H Okabe, Y Uji, K Netsu and A Noma

We automated a kinetic procedure for determining amylase isoenzymes in serum and urine samples. We used 4-nitro-phenylmaltoheptaoside as substrate and a selective amylase inhibitor with the Abbott-VP bichromatic system. By use of the maximum differences between pancreatic (P) and salivary (S) amylase activities remaining after inhibition by the selective inhibitor and by use of the linear range, a one-point standard method for calibration is proposed for determining amylase activities between about 50 and 1500 U/L when the P/S ratio exceeds 0.2. Results correlated well with those by electrophoresis and the Phadebas method (r = 0.99 for both pancreatic and salivary amylase). Reproducibilities (CVs) were 1.5% to 5.5% for pancreatic amylase and 1.4% to 3.3% for salivary amylase in serum, 0.8% to 2.0% for pancreatic amylase and 0.8% to 2.3% for salivary amylase in urine.


The following articles in journals at HighWire Press have cited this article:


Home page
 ANN INTERN MEDHome page
L. L. HUMPHRIES, L. J. ADAMS, J. H. ECKFELDT, M. D. LEVITT, and C. J. McCLAIN
Hyperamylasemia in Patients with Eating Disorders
Ann Intern Med, January 1, 1987; 106(1): 50 - 52.
[Abstract] [PDF]

Home page
 ANN INTERN MEDHome page
J. H. ECKFELDT, J. W. LEATHERMAN, and M. D. LEVITT
High Prevalence of Hyperamylasemia in Patients with Acidemia
Ann Intern Med, March 1, 1986; 104(3): 362 - 363.
[Abstract] [PDF]

Home page
 Arch Intern MedHome page
J. H. Eckfeldt and M. J. Kershaw
Hyperamylasemia Following Methyl Alcohol Intoxication: Source and Significance
Arch Intern Med, January 1, 1986; 146(1): 193 - 194.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1984 by the American Association for Clinical Chemistry.