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Clinical Chemistry, Vol 30, 1246-1249, Copyright © 1984 by American Association for Clinical Chemistry
T Olsson, V Kostulas and H Link
To demonstrate oligoclonal IgG bands (I) in unconcentrated cerebrospinal fluid, we used isoelectric focusing in agarose followed by protein transfer to cellulose nitrate membrane, double-antibody peroxidase labeling, and avidin-biotin amplification. I can be reliably seen after isoelectric focusing of 5-microL specimens containing 125 ng of IgG (25 mg/L). Thus the technique is more sensitive than others (e.g., silver staining) and more reliable than radioimmunofixation. When we used this technique with fluids from 62 patients with multiple sclerosis and infectious disease of the central nervous system, 84% displayed I, a percentage not increased when the same specimens were concentrated to 3.5 g of IgG per liter, examined by agarose isoelectric focusing, and stained with Coomassie Blue. Results for 53 patients with tension headache and psychoneurosis were all negative. By obviating the need to concentrate samples of cerebrospinal fluid the present method is a useful, sensitive alternative for demonstrating I.
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  V Zipoli, B Hakiki, E Portaccio, F Lolli, G Siracusa, M Giannini, L Pantoni, F Pescini, S Sorbi, and M. Amato The contribution of cerebrospinal fluid oligoclonal bands to the early diagnosis of multiple sclerosis Multiple Sclerosis, April 1, 2009; 15(4): 472 - 478. [Abstract] [PDF]
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M Soderstrom, J Hillert, J Link, V Navikas, S Fredrikson, and H Link Expression of IFN-{gamma}, IL-4, and TGF-{beta} in multiple sclerosis in relation to HLA-Dw2 phenotype and stage of disease Multiple Sclerosis, November 1, 1995; 1(3): 173 - 180. [Abstract] [PDF]
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 F. T. Sellebjerg, J. L. Frederiksen, and T. Olsson Anti--Myelin Basic Protein and Anti--Proteolipid Protein Antibody-Secreting Cells in the Cerebrospinal Fluid of Patients With Acute Optic Neuritis Arch Neurol, October 1, 1994; 51(10): 1032 - 1036. [Abstract] [PDF]
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M. Cruz, Y.-P. Jiang, J. Ernerudh, G. Solders, T. Olsson, P.-O. Osterman, and H. Link Antibodies to Myelin-Associated Glycoprotein Are Found in Cerebrospinal Fluid in Polyneuropathy Associated With Monoclonal Serum IgM Arch Neurol, January 1, 1991; 48(1): 66 - 70. [Abstract] [PDF]
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S. Fredrikson, J. Ernerudh, T. Olsson, P. Forsberg, and H. Link Mononuclear Cell Types in Cerebrospinal Fluid and Blood of Patients With Multiple Sclerosis: Quantitation by Immunoenzyme Microassay With Panel of Monoclonal Antibodies Arch Neurol, April 1, 1989; 46(4): 372 - 375. [Abstract] [PDF]
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V. K. Kostulas, H. Link, and A.-K. Lefvert Oligoclonal IgG Bands in Cerebrospinal Fluid: Principles for Demonstration and Interpretation Based on Findings in 1114 Neurological Patients Arch Neurol, October 1, 1987; 44(10): 1041 - 1044. [Abstract] [PDF]
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J. Ernerudh, T. Olsson, G. Berlin, and H. von Schenck Cerebrospinal Fluid Immunoglobulins and {beta}2-Microglobulin in Lymphoproliferative and Other Neoplastic Diseases of the Central Nervous System Arch Neurol, September 1, 1987; 44(9): 915 - 920. [Abstract] [PDF]
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V. K. Kostulas, A. Henriksson, and H. Link Monosymptomatic Sensory Symptoms and Cerebrospinal Fluid Immunoglobulin Levels in Relation to Multiple Sclerosis Arch Neurol, May 1, 1986; 43(5): 447 - 451. [Abstract] [PDF]
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