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Clinical Chemistry 30: 1344-1347, 1984;
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Clinical Chemistry, Vol 30, 1344-1347, Copyright © 1984 by American Association for Clinical Chemistry

Improved determination of cytochrome b5 in human erythrocytes

A Kaftory and E Hegesh

A sensitive, precise enzymic/spectrophotometric method for determining cytochrome b5 in small amounts of blood is described. Mean values for healthy individuals, ages 20 to 70 years, were 0.26 (SD 0.03) mumol per liter of erythrocytes or 0.87 (SD 0.14) nmol per gram of hemoglobin. We believe the assay is preferable to methods described hitherto, primarily because of its high sensitivity.




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Copyright © 1984 by the American Association for Clinical Chemistry.