Clinical Chemistry
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Clinical Chemistry 30: 1361-1365, 1984;
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Clinical Chemistry, Vol 30, 1361-1365, Copyright © 1984 by American Association for Clinical Chemistry

Radioimmunoassay of aspartate aminotransferase isoenzymes in human serum

FY Leung, AE Niblock and AR Henderson

We describe the development of a sensitive, specific radioimmunoassay for the cytoplasmic and mitochondrial isoenzymes of human aspartate aminotransferase (L-aspartate:2-oxoglutarate aminotransferase; EC 2.6.1.1). Isoenzymes from human heart tissue were purified to homogeneity and used to raise high-titer antisera in rabbits. We partly purified the antisera by selective column chromatography. The Bolton- Hunter reagent was used to radioiodinate the isoenzymes. The assay requires 100 microL of serum, includes a solid-phase second-antibody separation, and can be completed in less than 3 h. There was no cross reactivity between the two isoenzymes. As little as 5 micrograms (50 pmol) of each aspartate aminotransferase can be measured per liter of serum.




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Copyright © 1984 by the American Association for Clinical Chemistry.