Clinical Chemistry
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Clinical Chemistry 30: 1399-1401, 1984;
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Clinical Chemistry, Vol 30, 1399-1401, Copyright © 1984 by American Association for Clinical Chemistry

Diagnostic efficacy of a new enzyme immunoassay for creatine kinase MB isoenzyme

JJ Fenton, S Brunstetter, WC Gordon, DF Rippe and ML Bell

A new commercial enzyme immunoassay kit for quantification of creatine kinase-MB (CK-MB) isoenzyme was compared with its electrophoretic determination with respect to efficacy in diagnosis of acute myocardial infarction. Enzygnost CK-MB (Behring Diagnostics) is a solid-phase "sandwich"-type enzyme immunoassay with antibodies to the B-subunit coated on plastic tubes and peroxidase-conjugated antibodies to the M- subunit added after incubation with sample. This kit is designed to measure only CK-MB and not CK-MM, CK-BB, adenylate kinase, or atypical CK molecules. The linear-regression equation comparing the two methods was: Enzygnost = 0.98 . electrophoresis - 0.72, with a correlation coefficient of r = 0.967 (n = 143). For 51 patients admitted for diagnosis of possible acute myocardial infarction, the Enzygnost kit achieved 100% sensitivity, specificity, and efficiency in predicting the correct diagnosis. Corresponding values for the electrophoretic assay were: 95.5% sensitivity, 93.1% specificity, and 94.1% efficiency. We conclude that this kit method provides an excellent alternative to electrophoresis.


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A. Chiu, W.-K. Chan, S.-H. Cheng, C.-K. Leung, and C.-H. Choi
Troponin-I, myoglobin, and mass concentration of creatine kinase-MB in acute myocardial infarction
QJM, December 1, 1999; 92(12): 711 - 718.
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Copyright © 1984 by the American Association for Clinical Chemistry.