Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 30: 1462-1466, 1984;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rosenberg, J. S.
Right arrow Articles by Wilding, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rosenberg, J. S.
Right arrow Articles by Wilding, P.

Clinical Chemistry, Vol 30, 1462-1466, Copyright © 1984 by American Association for Clinical Chemistry

Immunogold staining: adaptation of a cell-labeling system for analysis of human leukocyte subsets

JS Rosenberg, E Weiss and P Wilding

We have assessed the Immunogold Cell-Labeling System (IGS) for potential use in the clinical laboratory. In this technique, cell suspensions incubated with monoclonal mouse antibodies are reacted with anti-mouse antibodies labeled with colloidal gold. Surface marker cells, bearing dark blue-black granules, are easily distinguished by light microscopy. The percentages of T cells, T cell subsets, B cells, monocytes, or granulocytes identified by IGS corresponds with numbers obtained by flow cytometry analysis or immunofluorescence studies. Results by IGS and flow cytometry were similar for samples from patients with aberrant lymphocyte populations (e.g., leukemias) or from transplant recipients. IGS may thus be a useful diagnostic technique for studying neoplasias or other immunologically mediated disorders. This technique can also be used to characterize the surface phenotype of leukemic cell lines. The sensitivity and accuracy of IGS can be evaluated by measurements of different cell lines mixed in predetermined ratios.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1984 by the American Association for Clinical Chemistry.