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Clinical Chemistry 30: 1467-1472, 1984;
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Clinical Chemistry, Vol 30, 1467-1472, Copyright © 1984 by American Association for Clinical Chemistry

Application of the MAST Immunodiagnostic System to the determination of allergen-specific IgE

SP Miller, VA Marinkovich, DH Riege, WJ Sell, DL Baker, NT Eldredge, JW Dyminski, RG Hale, JM Peisach and JF Burd

The MAST Immunodiagnostic Test System was developed to provide a comprehensive, simple means for the in vitro measurement of multiple antigens or antibodies. The first commercial application of the MAST system incorporates several novel features for cost-effective diagnosis of IgE-mediated allergy in a clinical laboratory or a physician's office. The basis of the MAST system is a unique analytical test chamber, which contains cellulose thread as the solid-phase matrix and allows multiple test results from a single assay. This test chamber incorporates both positive and negative controls and requires no volume- dependent pipetting steps. Immunographic exposure onto high-speed Polaroid instant film allows for quantifying results with an automatic recording infrared-transmittance densitometer. Test results are easily interpreted by using a patient test record provided with the system. The MAST system greatly simplifies testing for allergen-specific IgE, while retaining specificity and sensitivity. Currently, with the MAST system one can simultaneously measure picomoles of allergen-specific IgE in up to 35 different allergen classes. In addition to allergy testing, the MAST technology is applicable to other immunodiagnostic profiles.


The following articles in journals at HighWire Press have cited this article:


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Am. J. Respir. Crit. Care Med.Home page
N. Hizawa, E. Yamaguchi, D. Takahashi, J. Nishihira, and M. Nishimura
Functional Polymorphisms in the Promoter Region of Macrophage Migration Inhibitory Factor and Atopy
Am. J. Respir. Crit. Care Med., May 1, 2004; 169(9): 1014 - 1018.
[Abstract] [Full Text] [PDF]




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Copyright © 1984 by the American Association for Clinical Chemistry.