Clinical Chemistry
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Clinical Chemistry 31: 112-114, 1985;
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Clinical Chemistry, Vol 31, 112-114, Copyright © 1985 by American Association for Clinical Chemistry

Rapid separation and identification of myoglobin and hemoglobin in urine by centrifugation through a microconcentrator membrane

MJ Kelner and NM Alexander

Urinary myoglobin (Mr = 16 500) is readily separated from hemoglobin (Mr = 64 000) by centrifugation through a microconcentrator membrane with a 30 000-Da cutoff. Myoglobin, but not hemoglobin, will pass through the membrane, after which each fraction may be separately analyzed by spectrophotometry. This technique is advantageous over direct spectrophotometry of urine because it is not restricted to analyzing the oxy forms of these hemoproteins and obviates the difficulty of discriminating small (3 nm) differences in their spectra. In addition, this method of separation is more complete than that attained by differential "salting out" with ammonium sulfate, and it is simpler than gel filtration or ultrafiltration under reduced pressure.


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D. S. Grover, M. G. Atta, J. A. Eustace, T. S. Kickler, and D. M. Fine
Lack of clinical utility of urine myoglobin detection by microconcentrator ultrafiltration in the diagnosis of rhabdomyolysis
Nephrol. Dial. Transplant., October 1, 2004; 19(10): 2634 - 2638.
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Copyright © 1985 by the American Association for Clinical Chemistry.