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Clinical Chemistry 31: 122-124, 1985;
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Clinical Chemistry, Vol 31, 122-124, Copyright © 1985 by American Association for Clinical Chemistry

Pitfalls in, and new methods for, the radioimmunoassay for digoxin in urine

MS O'Brien, JR Huston, RJ Reid and TP Gibson

Examining techniques for radioimmunoassay of digoxin in urine, we found that the apparent concentration of digoxin is affected by endogenous substances, independent of the concentration of digoxin itself. We describe a modification of the Becton Dickinson solid-phase RIA that makes it more nearly accurate and more easily performed, and obviates the endogenous interference. Digoxin concentrations from 5.0 to at least 1000 micrograms/L can be measured.




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Copyright © 1985 by the American Association for Clinical Chemistry.