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Clinical Chemistry 31: 124-126, 1985;
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Clinical Chemistry, Vol 31, 124-126, Copyright © 1985 by American Association for Clinical Chemistry

Improved determination of estriol-16 alpha-glucuronide in pregnancy urine by direct liquid chromatography with fluorescence detection

F Andreolini, C Borra, A Di Corcia, R Samperi and G Raponi

In this relatively simple, rapid assay of estriol-16 alpha-glucuronide in pregnancy urine, the urine sample is diluted 20-fold with phosphate buffer (pH 5.2) containing 360 mL of acetonitrile and 2 g of cetyltrimethylammonium bromide per liter, then directly injected into the chromatograph. A sample can be assayed within 14 min. Day-to-day CVs ranged from 2.3% at 45 mg/L to 2.9% at 4.8 mg/L. The limit of sensitivity is 0.4 mg/L. Results by the present method (y) correlated and compared very well with those by a method involving fractionation of estrogen conjugates and gas chromatography (x) for 24 samples of pregnancy urine (y = 1.09x + 0.303; r = 0.947). This assay is inexpensive and suitable for complete automation.




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Copyright © 1985 by the American Association for Clinical Chemistry.