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Clinical Chemistry 31: 46-49, 1985;
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Clinical Chemistry, Vol 31, 46-49, Copyright © 1985 by American Association for Clinical Chemistry

Pregnanediol-3 alpha-glucuronide measured in diluted urine by mass spectrometry with fast atom bombardment/negative-ion ionization

G Moneti, G Agati, MG Giovannini, M Pazzagli, R Salerno, G Messeri and M Serio

We describe a mass-spectrometric method based on the fast atom bombardment ionization technique in the negative-ion mode for measuring pregnanediol-3 alpha-glucuronide in diluted urine from women. The procedure requires addition of testosterone-17 beta-D-glucuronide (2.5 micrograms/25 microL) to the urine sample as internal standard, and the sample is added directly to the fast atom bombardment target with no further manipulation. We have assessed and evaluated the method by the traditional criteria of reliability.




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