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Clinical Chemistry 31: 50-53, 1985;
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Clinical Chemistry, Vol 31, 50-53, Copyright © 1985 by American Association for Clinical Chemistry

Direct measurement of antigens in serum by time-resolved fluoroimmunoassay

JE Kuo, KH Milby, WD Hinsberg 3d, PR Poole, VL McGuffin and RN Zare

A long-lived fluorescence label (Tb3+) has been attached to the antigen of interest by using a bifunctional chelating agent 1-(p- benzenediazonium)-EDTA. A nonequilibrium competitive-binding immunoassay protocol, in conjunction with time-resolved detection of the long-lived fluorescence label, allows the antigen to be analyzed directly in samples containing diluted human serum. Results obtained for immunoglobulin G with this simple and rapid procedure correlated well (r = 0.93) with those by a commercially available fluorescence immunoassay method.




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Copyright © 1985 by the American Association for Clinical Chemistry.