Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 31: 1779-1789, 1985;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Doumas, B. T.
Right arrow Articles by Hause, L. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Doumas, B. T.
Right arrow Articles by Hause, L. L.

Clinical Chemistry, Vol 31, 1779-1789, Copyright © 1985 by American Association for Clinical Chemistry

Candidate reference method for determination of total bilirubin in serum: development and validation

BT Doumas, PP Kwok-Cheung, BW Perry, B Jendrzejczak, RB McComb, R Schaffer and LL Hause

This candidate Reference Method for measuring total bilirubin in serum is based on the Jendrassik-Grof principle (Clin Chem 29: 297-301, 1983). Standard Reference Material no. 916 bilirubin (National Bureau of Standards) is used as the standard. Bilirubin standard solutions may be prepared either in human serum or in 40 g/L albumin solution (human or bovine), because we found the molar absorptivity of the azopigment at 598 nm to be identical in these media. The absorptivities of the unconjugated and conjugated azopigments appear to be identical, but the conjugated azopigment is completely hydrolyzed in the final reaction mixture. Bilirubin added to serum from adults or neonates was quantitatively accounted for. Interference by hemoglobin (up to 2 g/L), ascorbic acid (up to 20 mg/L), or zinc (at physiological concentrations) is negligible. Of the therapeutic drugs we tested, only L-dopa and alpha-methyldopa interfere. We established normal adult reference values for total bilirubin and examined the intraindividual variation in 19 subjects.


The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
S. F. Lo, B. Jendrzejczak, and B. T. Doumas
Total or Neonatal Bilirubin Assays in the Vitros 5,1 FS: Hemoglobin Interference, Hemolysis, Icterus Index
Clin. Chem., April 1, 2007; 53(4): 799 - 800.
[Full Text] [PDF]

Home page
 Arch. Dis. Child. Fetal Neonatal Ed.Home page
H T Ho, T K Ng, K C Tsui, and Y C Lo
Evaluation of a new transcutaneous bilirubinometer in Chinese newborns
Arch. Dis. Child. Fetal Neonatal Ed., November 1, 2006; 91(6): F434 - F438.
[Abstract] [Full Text] [PDF]

Home page
 PediatricsHome page
K. Grohmann, M. Roser, B. Rolinski, I. Kadow, C. Muller, A. Goerlach-Graw, M. Nauck, and H. Kuster
Bilirubin Measurement for Neonates: Comparison of 9 Frequently Used Methods
Pediatrics, April 1, 2006; 117(4): 1174 - 1183.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
R. Rej
Clinical Chemistry through Clinical Chemistry: A Journal Timeline
Clin. Chem., December 1, 2004; 50(12): 2415 - 2458.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
J. P. J. Ungerer, S. J. Southby, C. M. Florkowski, and P. M. George
Automated Measurement of Cerebrospinal Fluid Bilirubin in Suspected Subarachnoid Hemorrhage
Clin. Chem., October 1, 2004; 50(10): 1854 - 1856.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
S. F. Lo, B. T. Doumas, and E. R. Ashwood
Performance of Bilirubin Determinations in US Laboratories--Revisited
Clin. Chem., January 1, 2004; 50(1): 190 - 194.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
B. T. Doumas, F. Yein, B. Perry, B. Jendrzejczak, and A. Kessner
Determination of the Sum of Bilirubin Sugar Conjugates in Plasma by Bilirubin Oxidase
Clin. Chem., August 1, 1999; 45(8): 1255 - 1260.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
E. Moura, M. A. Noordermeer, N. Verhoeven, A. F.M. Verheul, and J. J.M. Marx
Iron Release From Human Monocytes After Erythrophagocytosis In Vitro: An Investigation in Normal Subjects and Hereditary Hemochromatosis Patients
Blood, October 1, 1998; 92(7): 2511 - 2519.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1985 by the American Association for Clinical Chemistry.