| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Clinical Chemistry, Vol 31, 1815-1819, Copyright © 1985 by American Association for Clinical Chemistry
BW Hollis and JL Napoli
We describe a faster, more-sensitive radioimmunoassay for vitamin D in plasma. Antibodies were generated in rabbits immunized with a new vitamin D analog, the 23,24,25,26,27-pentanor-C(22)-carboxylic acid of vitamin D, coupled directly with bovine serum albumin. After several months, Rivanol-treated sera from the rabbits contained high-titer antibodies, as determined by their abilities to bind 25-hydroxy- [3H]cholecalciferol. The antibody, used at a 1:15 000 final dilution, cross reacted equally with all cholecalciferol and ergocalciferol metabolites tested except 1,25-dihydroxycalciferol metabolites and the parent calciferols. 25-Hydroxycalciferol and similar forms were efficiently extracted from plasma or serum with acetonitrile. We separated bound from free 25-hydroxy-[3H]cholecalciferol by using a second antibody: goat antiserum to rabbit serum. The detection limit of the assay was 3.0 pg of 25-hydroxycholecalciferol and its equivalents per tube; thus only 1 microL of plasma is needed per assay tube. Results compared well with those from a liquid-chromatographic procedure involving specific ultraviolet detection of 25- hydroxycalciferol in plasma.
The following articles in journals at HighWire Press have cited this article:
|
|
 |
|
  J. E Zerwekh Blood biomarkers of vitamin D status Am. J. Clinical Nutrition, April 1, 2008; 87(4): 1087S - 1091S. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Singh, R. L. Taylor, G. S. Reddy, and S. K. G. Grebe C-3 Epimers Can Account for a Significant Proportion of Total Circulating 25-Hydroxyvitamin D in Infants, Complicating Accurate Measurement and Interpretation of Vitamin D Status J. Clin. Endocrinol. Metab., August 1, 2006; 91(8): 3055 - 3061. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. L. Lensmeyer, D. A. Wiebe, N. Binkley, and M. K. Drezner HPLC Method for 25-Hydroxyvitamin D Measurement: Comparison with Contemporary Assays Clin. Chem., June 1, 2006; 52(6): 1120 - 1126. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. W. Hollis Editorial: The Determination of Circulating 25-Hydroxyvitamin D: No Easy Task J. Clin. Endocrinol. Metab., July 1, 2004; 89(7): 3149 - 3151. [Full Text] [PDF]
|
|
|
|
|
|
N. Binkley, D. Krueger, C. S. Cowgill, L. Plum, E. Lake, K. E. Hansen, H. F. DeLuca, and M. K. Drezner Assay Variation Confounds the Diagnosis of Hypovitaminosis D: A Call for Standardization J. Clin. Endocrinol. Metab., July 1, 2004; 89(7): 3152 - 3157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Turpeinen, U. Hohenthal, and U.-H. Stenman Determination of 25-Hydroxyvitamin D in Serum by HPLC and Immunoassay Clin. Chem., September 1, 2003; 49(9): 1521 - 1524. [Full Text] [PDF]
|
|
|
|
|
|
B. W. Hollis Comparison of Commercially Available 125I-based RIA Methods for the Determination of Circulating 25-Hydroxyvitamin D Clin. Chem., October 1, 2000; 46(10): 1657 - 1661. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Goswami, N. Gupta, D. Goswami, R. K. Marwaha, N. Tandon, and N. Kochupillai Prevalence and significance of low 25-hydroxyvitamin D concentrations in healthy subjects in Delhi Am. J. Clinical Nutrition, August 1, 2000; 72(2): 472 - 475. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
