Clinical Chemistry
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Clinical Chemistry 31: 1833-1837, 1985;
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Clinical Chemistry, Vol 31, 1833-1837, Copyright © 1985 by American Association for Clinical Chemistry

Competitive idiotype--anti-idiotype immunoassay for adenosine deaminase binding protein in urine

RE Thompson, CR Hewitt, DJ Piper, WP Hansen, RH Rubin, NE Tolkoff-Rubin, MC Barrett and MJ Nelles

This competitive immunoassay, based on inhibition by antigen of the idiotype-anti-idiotype interaction, detects adenosine deaminase binding protein (ABP), and involves use of monoclonal anti-idiotype antibodies prepared to a monoclonal antibody specific for ABP. The conditions for this new type of competitive immunoassay are investigated. This competitive immunoassay is as sensitive and reproducible as an earlier described "sandwich"-type immunoassay for ABP (Clin Chem 31: 679-683, 1985). Evaluation of urine samples from normal subjects and from patients showed increased concentrations of ABP in patients with renal disease.




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Copyright © 1985 by the American Association for Clinical Chemistry.