Clinical Chemistry
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Clinical Chemistry 31: 1846-1848, 1985;
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Clinical Chemistry, Vol 31, 1846-1848, Copyright © 1985 by American Association for Clinical Chemistry

Facile radioimmunoassay of estradiol 3-sulfate in plasma during pregnancy

T Tanaka, N Suguro and A Kubodera

A facile, sensitive radioimmunoassay of estradiol 3-sulfate in pregnancy plasma was established. Highly specific antisera were raised in male rabbits against a conjugate of 6-oxoestradiol 3-sulfate-(O- carboxymethyl)oxime with bovine serum albumin. After purification of the antiserum, we coupled the crude globulin fraction to CNBr-activated Sepharose-4B and used this for direct radioimmunoassay of estradiol 3- sulfate in plasma without sample pre-treatment such as solvent- extraction or chromatography to remove endogenous interferences. Using this assay system, we determined concentrations of estradiol 3-sulfate in plasma from pregnant women. Concentrations of estradiol 3-sulfate in plasma increased in proportion to those of estradiol throughout gestation, but were about twice as high as those of unconjugated estradiol at each week of pregnancy.


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 EndocrinologyHome page
C. E. Wood, K. E. Gridley, and M. Keller-Wood
Biological Activity of 17{beta}-Estradiol-3-Sulfate in Ovine Fetal Plasma and Uptake in Fetal Brain
Endocrinology, February 1, 2003; 144(2): 599 - 604.
[Abstract] [Full Text] [PDF]


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Copyright © 1985 by the American Association for Clinical Chemistry.