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Clinical Chemistry 31: 1893-1895, 1985;
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Clinical Chemistry, Vol 31, 1893-1895, Copyright © 1985 by American Association for Clinical Chemistry

Polyacrylamide gel electrophoresis in quantification of high-density lipoprotein cholesterol

D Roche, V Atger, NT Le Quang, A Girard and OG Ekindjian

We evaluated a method for quantifying high-density lipoprotein cholesterol in plasma, based on electrophoretic migration of the prestained (with Sudan Black III) sample through a discontinuous polyacrylamide++ gel and densitometric integration of the stain associated with each class of lipoprotein. With this method, operations can be carried out on all types of lipoproteins over a broad range of concentrations. Overloading with very-low and low-density lipoproteins did not affect reliability within a wide range of HDL concentrations (0.45 to 16.60 mmol/L). Results for 22 individual plasma samples from normal and dyslipemic subjects correlated well with those by ultracentrifugal analysis (r=0.96; Student's t= 0.90, p > 0.30). We conclude that this method is reliable, sensitive, and accurate, It may be used for simultaneously typing dyslipoproteinemias and assaying HDL cholesterol.




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Copyright © 1985 by the American Association for Clinical Chemistry.