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Clinical Chemistry 31: 257-260, 1985;
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Clinical Chemistry, Vol 31, 257-260, Copyright © 1985 by American Association for Clinical Chemistry

Measurement of lipase activity by a differential pH technique

F Ceriotti, PA Bonini, M Murone, L Barenghi, M Luzzana, A Mosca, M Ripamonti and L Rossi-Bernardi

This is a new electrochemical method for determination of lipase activity in biological fluids, including serum, plasma, and duodenal juice. Advantages of turbidimetric methods--short reaction time, and small sample and reagent volumes--are combined with those of titrimetric methods: measurement of absolute activity (i.e., no standardization required), saturated substrate conditions, and direct measurement of reaction products. The proposed method is easy, inexpensive, and takes only 3 min. Precision is good: CV = 3.74% within day and 7.3% between days at the clinical-decision concentration, CV = 1.86% within day and 4.65% between days for above-normal lipase activities. The standard curve is linear up to 4500 U/L. Results (y) correlate well with those by turbidimetry (x): y = 0.9287x - 65.3 (r = 0.9719). Reference values are between 0 and 130 U/L.




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Copyright © 1985 by the American Association for Clinical Chemistry.