Clinical Chemistry
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Clinical Chemistry 31: 276-278, 1985;
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Clinical Chemistry, Vol 31, 276-278, Copyright © 1985 by American Association for Clinical Chemistry

Stability of bombesin in serum, plasma, urine, and culture media

K Tateishi, GG Klee, JM Cunningham and VA Lennon

Using a radioimmunoassay, we studied the stability of bombesin added to pooled human serum, plasma, urine, and two culture media (RPMI-1640 and M199). Bombesin was stable in serum for 5 h at 4 degrees C and 2 h at 21 degrees C. However, in plasma, about half was no longer detected by 5 h at 4 degrees C, and this decline was unaffected by added aprotinin. Bombesin was stable in urine for 24 h at 4 degrees C and for 2 h at 21 degrees C. When incubated in the culture media at 37 degrees C for 60 min, no loss was detected. Bombesin-like immunoreactivity was detectable in the serum of 22 mice bearing tumors established from biopsied small-cell lung carcinomas, two of 12 mice bearing tumors established from other malignancies and zero of nine control mice. In contrast, immunoreactivity to this analyte was detected in urine specimens from all mice except for one bearing a non-small-cell tumor. The concentrations detected in serum and urine were uncorrelated.


The following articles in journals at HighWire Press have cited this article:


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 Am. J. Respir. Crit. Care Med.Home page
A. Cullen, L. J. Van Marter, E. N. Allred, M. Moore, R. B. Parad, and M. E. Sunday
Urine Bombesin-like Peptide Elevation Precedes Clinical Evidence of Bronchopulmonary Dysplasia
Am. J. Respir. Crit. Care Med., April 15, 2002; 165(8): 1093 - 1097.
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Copyright © 1985 by the American Association for Clinical Chemistry.