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Clinical Chemistry 31: 279-281, 1985;
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Clinical Chemistry, Vol 31, 279-281, Copyright © 1985 by American Association for Clinical Chemistry

Carboxyhemoglobin determination by second-derivative spectroscopy

J Parks and HG Worth

In this procedure hemoglobin is converted to its reduced form and the magnitude of the zero-order spectral shift of the reduced hemoglobin peak at 430 nm to the carboxyhemoglobin peak at 418 nm is determined by second-derivative spectrum analysis. The method is simple, straightforward to set up, and rapid. A result may be obtained within 15 min of receiving the sample. It is sufficiently sensitive to differentiate carboxyhemoglobin concentration in the blood of smokers and nonsmokers.




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Copyright © 1985 by the American Association for Clinical Chemistry.