Clinical Chemistry
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Clinical Chemistry 31: 289-292, 1985;
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Clinical Chemistry, Vol 31, 289-292, Copyright © 1985 by American Association for Clinical Chemistry

Detection of thyroid disorders by use of basal thyrotropin values determined with an optimized "sandwich" enzyme immunoassay

C Bernutz, M Kewenig, K Horn and CR Pickardt

To measure the concentrations of thyrotropin (thyroid-stimulating hormone), we used the components of a commercially available two-step "sandwich" enzyme immunoassay (Enzymun-Test TSH, Boehringer Mannheim) based on the specific binding of the beta-subunit of thyrotropin by monoclonal antibodies coated on polystyrene tubes. By modifying the original assay protocol, we lowered the limit of detection to 0.18 milli-int. units/L, using a total incubation period of 22 h. With this modification we could differentiate between patients responsive to administration of thyroliberin (thyrotropin-releasing factor) and those who were non-responders, by measuring only the basal concentration of thyrotropin. Furthermore, we demonstrated a correlation between the basal concentration of thyrotropin and its increase after administration of thyroliberin (r = 0.77, n = 48).




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Copyright © 1985 by the American Association for Clinical Chemistry.