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Clinical Chemistry 31: 306-308, 1985;
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Clinical Chemistry, Vol 31, 306-308, Copyright © 1985 by American Association for Clinical Chemistry

How certain drugs interfere negatively with the Jaffe reaction for creatinine

MH Kroll, L Nealon, MA Vogel and RJ Elin

Phenacemide and cephalothin may interfere either positively or negatively with the determination of creatinine by the Jaffe reaction, depending on the analytical system. The drug-picrate chromophores maximally absorb within the first minute of reaction (21 s for phenacemide, 45 s for cephalothin), after which the absorbances decrease. Thus, these drugs negatively interfere in analytical systems involving kinetic measurements when the time interval of measurement includes the period of decreasing absorbance.




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Copyright © 1985 by the American Association for Clinical Chemistry.