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Clinical Chemistry 31: 391-396, 1985;
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Clinical Chemistry, Vol 31, 391-396, Copyright © 1985 by American Association for Clinical Chemistry

Radioimmunoassay for arylsulfatase A in urine

PM Laidler, KW Ryder, MR Glick, TO Oei and RL Van Etten

Purified arylsulfatase A (EC 3.1.6.1) from human urine was radioiodinated under conditions that caused no significant loss of antigenic activity. We used this labeled arylsulfatase A (specific radioactivity 4-7.5 Ci/g) together with nonlabeled enzyme and rabbit antiserum produced against homogeneous enzyme to develop a radioimmunoassay for arylsulfatase A in urine. A solid-phase, second- antibody technique (Immunobead Second Antibody; Bio-Rad Laboratories) was used to separate free enzyme from antigen-antibody complexes. The working range of the assay was 0.1-4.0 ng of enzyme; within- and between-assay CVs were around 10%, and the analytical recovery was 105.5% (SD 7.7%). The lower limit of detection was 0.08 ng of arysulfatase A per assay, substantially less than that of typical activity-based assays. Over a wide range of urinary arylsulfatase A activities, results by this method agreed well (r = 0.99) with those obtained by activity assays. We measured the enzyme in urines of 59 healthy volunteers and 92 patients with different diseases, including a group of colorectal cancer cases, to determine whether this could serve as a reliable marker for cancer of the colon; however, urinary excretion of arylsulfatase A by most patients with colon cancer was within normal limits.


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J.E. Bright, R.H. Inns, N.J. Tuckwell, and T.C. Marrs
The Effect of Storage upon Cyanide in Blood Samples
Human and Experimental Toxicology, January 1, 1990; 9(3): 125 - 129.
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Copyright © 1985 by the American Association for Clinical Chemistry.