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Clinical Chemistry 31: 441-444, 1985;
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Clinical Chemistry, Vol 31, 441-444, Copyright © 1985 by American Association for Clinical Chemistry

Choriogonadotropin measured with the Tandem-E immunoenzymetric assay system

JL Bock, J Furgiuele and JC Segen

We evaluated the Hybritech Tandem-E procedure for quantifying choriogonadotropin (hCG) in human serum. In this "sandwich"-type assay, two monoclonal antibodies directed against different regions of the hCG molecule are used, one coated on a plastic bead, the second conjugated to alkaline phosphatase. The assay can detect as little as 1.0 int. unit of the hormone per liter, shows a linear response up to at least 200 int. units/L, and has good precision. By prolonging the incubations for formation of the sandwich and for substrate hydrolysis, one can achieve higher sensitivity at the expense of a narrower linear range. Correlation with a conventional radioimmunoassay for the beta subunit of hCG was generally excellent, but in one instance the Tandem-E gave an apparently false positive result.




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Copyright © 1985 by the American Association for Clinical Chemistry.