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Clinical Chemistry 31: 551-555, 1985;
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Clinical Chemistry, Vol 31, 551-555, Copyright © 1985 by American Association for Clinical Chemistry

Simultaneous determination of trace elements in lavage fluids from human bronchial alveoli by energy dispersive x-ray fluorescence. 1: Technique and determination of the normal reference interval

EA Maier, F Rastegar, R Heimburger, C Ruch, A Pelletier, A Maier and MJ Leroy

We have applied energy dispersive x-ray fluorescence to the analysis of broncho-alveolar lavage fluids, to determine the concentration of several elements simultaneously with manipulation of the sample minimized. A 20-microL sample of a homogenized lavage fluid, to which two internal standards (containing Y and V) have been added, is deposited on a polypropylene film, 4 microns in thickness, and analyzed by x-ray fluorescence. We report the analytical procedure, the detection limits, and the reproducibility. The normal concentration range for trace elements is reported for a selected population. The simplicity and rapidity of the method, and the possibility of automating measurements, make this procedure suitable for screening large numbers of lavage specimens and to establish the diagnosis of some pneumoconioses.


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A. Mutti, M. Corradi, M. Goldoni, M. V. Vettori, A. Bernard, and P. Apostoli
Exhaled Metallic Elements and Serum Pneumoproteins in Asymptomatic Smokers and Patients With COPD or Asthma
Chest, May 1, 2006; 129(5): 1288 - 1297.
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Copyright © 1985 by the American Association for Clinical Chemistry.