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Clinical Chemistry 31: 605-608, 1985;
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Clinical Chemistry, Vol 31, 605-608, Copyright © 1985 by American Association for Clinical Chemistry

Evaluation and comparison of a radiative energy attenuation method for determining cholesterol in serum

PL Cary, CA Johnson, PD Whitter and JW Parker

Determination of cholesterol by a radiative energy attenuation (REA) technique was evaluated and compared with results obtained by the Boehringer Mannheim High Performance Cholesterol Assay and the Du Pont aca. Within-assay and between-assay CVs for the REA method, for two sets of controls, were both less than 5%. We observed no interference with lipemic samples. Analytical recovery averaged 102.8%. We used all three methods for parallel determinations of 217 patients' samples containing a wide range of cholesterol concentrations. Linear regression analysis of the REA results vs those of the comparison methods were as follows: REA = 1.03 Boehringer - 0.072 (r = 0.993) and REA = 1.02 aca - 0.048 (r = 0.995). We also discuss bilirubin interference with the REA method for cholesterol.




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Copyright © 1985 by the American Association for Clinical Chemistry.