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Clinical Chemistry, Vol 31, 645-646, Copyright © 1985 by American Association for Clinical Chemistry
JE Buttery and BR Chamberlain
The absorbance difference measured when angiotensin-converting enzyme (EC 3.4.15.1) hydrolyzes the substrate N-[3-(2-furyl)acryloyl]-L- phenylalanylglycylglycine is the basis for measuring its activity. We show this difference to be instrument dependent, and describe a method for deriving it that is applicable to manual or automated procedures.
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