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Clinical Chemistry 31: 727-731, 1985;
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Clinical Chemistry, Vol 31, 727-731, Copyright © 1985 by American Association for Clinical Chemistry

Liquid-chromatographic measurement of purine nucleotides in blood cells

R Boulieu, C Bory and C Gonnet

In this anion-exchange "high-performance" liquid-chromatographic method of analysis for purine nucleotides, the nucleotides are separated with high efficiency and selectivity on a weak anion exchanger (Hypersil APS 2, 3-micron particle size) by elution with a gradient of eluent pH and concentration. Applying this method to analysis for these compounds in human blood cells, we determined them in a patient with adenosine deaminase deficiency who was treated with a bone-marrow transplantation, finding that the transplantation did not entirely correct the patient's abnormalities of purine metabolism.




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Copyright © 1985 by the American Association for Clinical Chemistry.