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Clinical Chemistry 31: 750-753, 1985;
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Clinical Chemistry, Vol 31, 750-753, Copyright © 1985 by American Association for Clinical Chemistry

Enzyme immunoassay of free thyroxin in dried blood samples on filter paper

N Hata, K Miyai, M Ito, Y Endo, Y Iijimi, H Mizuta, N Amino, O Nose and T Harada

We describe a double-antibody enzyme immunoassay for determination of free thyroxin (FT4) in dried blood samples on filter paper, with use of a T4-beta-D-galactosidase complex. The measurable range of FT4 concentration in two 3-mm blood discs, each of which contained about 2.7 microL of blood, was 1.9 to 93 ng/L, as determined by comparison with concentrations of FT4 in known serum standards. FT4 in blood samples dried on filter paper was stable for at least four weeks when kept dry at -20 degrees C, room temperature, or 37 degrees C. The mean coefficients of variation were 7.6% (within assay) and 6.4% (between assays). Results for FT4 by this method correlated well with those for serum determined by radioimmunoassay (r = 0.98). The proposed method can be used to differentiate persons with hyper- and hypothyroidism from normal subjects and those with abnormal concentrations of thyroxin- binding globulin. The procedure seems suited for screening studies.




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Copyright © 1985 by the American Association for Clinical Chemistry.