Clinical Chemistry
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Clinical Chemistry 31: 826-827, 1985;
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Clinical Chemistry, Vol 31, 826-827, Copyright © 1985 by American Association for Clinical Chemistry

Fluorometric assay of alpha-L-iduronidase in serum for detection of affected and carrier animals in a canine model of mucopolysaccharidosis I

RM Shull and NE Hastings

We investigated measuring serum alpha-L-iduronidase (EC 3.2.1.76) by a sensitive fluorometric assay in 28 members of a canine family with mucopolysaccharidosis I. If assayed on the day of collection, serum was an acceptable specimen for identifying affected, carrier, and normal dogs. The overall correlation (r) between iduronidase activity in serum and mononuclear leukocytes was 0.966. However, iduronidase was extremely labile in serum refrigerated or frozen for 48 h.


The following articles in journals at HighWire Press have cited this article:


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 Clin. Chem.Home page
N. A. Chamoles, M. Blanco, and D. Gaggioli
Diagnosis of {{alpha}}-L-Iduronidase Deficiency in Dried Blood Spots on Filter Paper: The Possibility of Newborn Diagnosis
Clin. Chem., April 1, 2001; 47(4): 780 - 781.
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