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Clinical Chemistry 31: 871-872, 1985;
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Clinical Chemistry, Vol 31, 871-872, Copyright © 1985 by American Association for Clinical Chemistry

Stability of vitamin A in frozen sera

WJ Driskell, AD Lackey, JS Hewett and MM Bashor

We compared values for vitamin A measured in fresh sera with values obtained after storage at -20 degrees C for five to eight years. Loss of vitamin A from long-stored sera during the extraction step was eliminated by adding ascorbic acid to the extraction solvent, ethanol. Retinol-binding protein was also measured in the stored sera. Not only did vitamin A values remain stable during the years of storage, but also the correlation between concentrations of vitamin A and retinol- binding protein in the stored sera was typical of that observed with fresh sera.


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Copyright © 1985 by the American Association for Clinical Chemistry.